Description
Lactobacilli MRS Broth
Lactobacilli MRS Broth
Lactobacilli MRS Broth, also referred to as Lactobacillus MRS Broth or LMRS Broth, is a liquid medium used for the selective isolation and enumeration of Lactobacilli from various sources which include feces and dairy. Beef Extract and Yeast Extract provide nitrogenous sources, carbohydrates, vitamins, and minerals. Dextrose is the main fermentable carbohydrate. Sodium Acetate and Ammonium Citrate serve as both selective agents and energy sources. Potassium Phosphate acts as pH buffer, while Magnesium and Manganese Sulfates provide cations essential for bacterial metabolism. Tween 80 is a surfactant to facilitate nutrient uptake by the Lactobacilli. There is a choice of 3 sizes – 500g, 2kg, and 10kg.
PREPARATION: Mix 55 grams of the medium in one liter of purified water until evenly dispersed. Heat with repeated stirring to dissolve completely. Autoclave at 121°C for 15 minutes.
Additional Literature:
L12-104 Product Information
L12-104 Safety Data Sheet
