Description
Hsu’s Lactobacillus Pediococcus Medium
Hsu’s Lactobacillus Pediococcus Medium
Hsu’s Lactobacillus Pediococcus Medium is a selective semi-solid medium that allows for the detection of Lactobacillus and Pediococcus species, while restricting the growth of culture yeast. The inclusion of agar at 2% makes it a semisolid medium, which offers a means of identifying lactic acid bacteria to the genus level on the basis of colony morphology. There is a choice of 3 sizes – 500g, 2kg, and 10kg.
PREPARATION: Mix 7 grams of the medium in 100 mL of purified water until evenly dispersed. Heat with repeated stirring and boil for 2-3 minutes to dissolve completely. Autoclaving is not necessary. Cool medium to approx. 45-50°C in a water bath and transfer aliquots of 17 mL to sterile 16x150mm test tubes, leaving ~ 1 cm (or equivalent to desired sample volume) of headspace. Apply caps and screw tightly. Alternatively, 1.5 to 2.0 grams of agar may be added in addition to the 7 grams of medium in order to produce solid media. If this is the desired application, pour the prepared medium into sterile petri dishes and test according to desired protocol.
Additional Literature:
H08-107 Product Information
H08-107 Safety Data Sheet
